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Transcription Activator-Like Effector Nucleases - TALENs

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In this video we discuss a type of synthetic nuclease, namely the TALEN, standing for transcription activator-like effector nucleases.
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Text Comments (38)
Anna Kassis (1 month ago)
That's the best explanation ever, (Fok1)
meta morfoz (1 month ago)
I cant focus on video because of your pronunciation
MrPelafio (1 month ago)
I was just about to google difference between TALEN and ZFN, when at the very end you explained it. Wonderful!
Aroni Mitra (3 months ago)
RVDs in a particular TALE recognizes different bases. So change in RVDs results in change in sequence specificity. 9 TALEs don't copolymerize. Only two TALEs with different RVDs bind to two adjacent sides of Fok1 recognition sequence. You're explanation is pretty much wrong.
Aroni Mitra (3 months ago)
Nidhya Joseph TALES from Xanthomonas has a DNA binding domain and an activator domain which allows it to regulate the activity of certain genes in it's host. The DNA binding domain has 30 repeats of 33-35 amino acid residues. Out of these 35 residues, residue number 12 and 13 are variable in the repeats and thus they are called repeat variable diresidue. These two amino acids are responsible for recognition of a single nucleotide in DNA. Amino acids NI at RVDs recognizes adenine, NG recognizes thymine, HD recognizes cytosine and NN recognizes adenine or guanine. So if you know the sequence of your DNA then you can design TALENS that bind to your specific sequence by changing the amino acids at 12 and 13th position of each repeat. In this way the talen can bind to your sequence and the DNA cleavage domain of Fok1 fused to the DNA binding domain of TALE can cleave the DNA. Generally two TALENS have to be coexpressed in a cell, one that binds upstream of the cleavage site and one that binds downstream. When the two will bind, two Fok1 domains come in vicinity and cleave the DNA molecule. The cleaved DNA can now be joined by non-homologous end joining which is an error prone double stranded break repair process , leading to incorporation of mutations in your desired sequence of interest.
Nidhya Joseph (3 months ago)
Can you please elaborate? I did not get what exactly was stated wrong here.. Also how are both the TALEs and REN -eg. Fok 1 made so specific for a particular sequence? Both the TALES are different sequences right? Are there any chances of both these TAL effectors binding to each other..?
Angelo Orsini (3 months ago)
The best one on youtube. Thanks man!
Angelo Orsini (3 months ago)
thx
sarah Brm (4 months ago)
the best* one ..
condimentado (11 months ago)
BEST EXPLANATION EVER!!!!
Rebecca Maysonet (11 months ago)
this is the best explanation I've seen. Thanks!
Jennifer M (1 year ago)
Great explanation....thanks!
Benjie Hutchison (1 year ago)
It's not FOKL, it's FOK1 The bacteria is also called Xanthomonas, not what was described in the video
Neo 1000 (1 year ago)
Thank you very much
Alberto Bray (1 year ago)
AWSOME explanation!!! Thank you so much
Eathaar E (1 year ago)
nine-tails hah
changachangueria (1 year ago)
WOW!! nice explanation!! TY so much.. great job
Harry Li (1 year ago)
Best explanation ever! Thanks so much for your clear explanations and visual demonstrations!
Daniel Ivanov (1 year ago)
Thank you !
Mariah Foley (1 year ago)
What about RVDs
sapastje (1 year ago)
helped a lot, thx!
Christina (1 year ago)
THANKS!
Chutimon Fang (1 year ago)
Thank you so much this helps me a lot.
Gasper Kosmac (2 years ago)
THANK YOU
maha chris (2 years ago)
Thanks , you made it look so simple.
Benji Price (2 years ago)
are you talking about restriction enzymes or is it something completely different? please do answer me, how about you @ozan ATASOY
Johnson Qu (2 years ago)
very nice
Ozan ATASOY (2 years ago)
Thank you friend. As a virologist it's very simple and informative explanation for me.
Paula Dalcin Martins (2 years ago)
Great explanation!
Harley Jerram (2 years ago)
Thank you, really nice video!
Terea Stetina (3 years ago)
Thanks, this is a great explanation. Xanthomonas FOK1
Cheng Vincent (3 years ago)
Great explanation, I am not sure that is FokL or Fok I
ardalan mansouri (2 years ago)
It is actually Fok1 (or capital i as a Greek number instead of "1"). For more information regarding its discovery please go to the following link published by the first researcher discovered it: http://www.ncbi.nlm.nih.gov/pubmed/6282705

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