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Transcription Activator-Like Effector Nucleases - TALENs

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Оценок: 368 | Просмотров: 29092
In this video we discuss a type of synthetic nuclease, namely the TALEN, standing for transcription activator-like effector nucleases.
Категория: Образование
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Текстовые комментарии (35)
Cannizzo Sam (23 часа назад)
* FOK1 not FOKL
Alexandra Herrarte (20 дней назад)
skip to 2:56, first few mins are bogus
Anna Kassis (6 месяцев назад)
That's the best explanation ever, (Fok1)
meta morfoz (6 месяцев назад)
I cant focus on video because of your pronunciation
MrPelafio (6 месяцев назад)
I was just about to google difference between TALEN and ZFN, when at the very end you explained it. Wonderful!
Aroni Mitra (8 месяцев назад)
RVDs in a particular TALE recognizes different bases. So change in RVDs results in change in sequence specificity. 9 TALEs don't copolymerize. Only two TALEs with different RVDs bind to two adjacent sides of Fok1 recognition sequence. You're explanation is pretty much wrong.
Aroni Mitra (8 месяцев назад)
Nidhya Joseph TALES from Xanthomonas has a DNA binding domain and an activator domain which allows it to regulate the activity of certain genes in it's host. The DNA binding domain has 30 repeats of 33-35 amino acid residues. Out of these 35 residues, residue number 12 and 13 are variable in the repeats and thus they are called repeat variable diresidue. These two amino acids are responsible for recognition of a single nucleotide in DNA. Amino acids NI at RVDs recognizes adenine, NG recognizes thymine, HD recognizes cytosine and NN recognizes adenine or guanine. So if you know the sequence of your DNA then you can design TALENS that bind to your specific sequence by changing the amino acids at 12 and 13th position of each repeat. In this way the talen can bind to your sequence and the DNA cleavage domain of Fok1 fused to the DNA binding domain of TALE can cleave the DNA. Generally two TALENS have to be coexpressed in a cell, one that binds upstream of the cleavage site and one that binds downstream. When the two will bind, two Fok1 domains come in vicinity and cleave the DNA molecule. The cleaved DNA can now be joined by non-homologous end joining which is an error prone double stranded break repair process , leading to incorporation of mutations in your desired sequence of interest.
Nidhya Joseph (8 месяцев назад)
Can you please elaborate? I did not get what exactly was stated wrong here.. Also how are both the TALEs and REN -eg. Fok 1 made so specific for a particular sequence? Both the TALES are different sequences right? Are there any chances of both these TAL effectors binding to each other..?
Angelo Orsini (8 месяцев назад)
The best one on youtube. Thanks man!
Angelo Orsini (8 месяцев назад)
sarah Brm (8 месяцев назад)
the best* one ..
condimentado (1 год назад)
Rebecca Maysonet (1 год назад)
this is the best explanation I've seen. Thanks!
Jennifer M (1 год назад)
Great explanation....thanks!
Benjie Hutchison (1 год назад)
It's not FOKL, it's FOK1 The bacteria is also called Xanthomonas, not what was described in the video
Neo 1000 (1 год назад)
Thank you very much
Alberto Bray (1 год назад)
AWSOME explanation!!! Thank you so much
Eathaar E (1 год назад)
nine-tails hah
changachangueria (1 год назад)
WOW!! nice explanation!! TY so much.. great job
Harry Li (1 год назад)
Best explanation ever! Thanks so much for your clear explanations and visual demonstrations!
Daniel Ivanov (1 год назад)
Thank you !
Mariah Foley (2 года назад)
What about RVDs
sapastje (2 года назад)
helped a lot, thx!
Christina (2 года назад)
Chutimon Fang (2 года назад)
Thank you so much this helps me a lot.
Gasper Kosmac (2 года назад)
maha chris (2 года назад)
Thanks , you made it look so simple.
Benji Price (2 года назад)
are you talking about restriction enzymes or is it something completely different? please do answer me, how about you @ozan ATASOY
Johnson Qu (2 года назад)
very nice
Ozan ATASOY (2 года назад)
Thank you friend. As a virologist it's very simple and informative explanation for me.
Paula Dalcin Martins (3 года назад)
Great explanation!
Harley Jerram (3 года назад)
Thank you, really nice video!
Terea Stetina (3 года назад)
Thanks, this is a great explanation. Xanthomonas FOK1
Cheng Vincent (3 года назад)
Great explanation, I am not sure that is FokL or Fok I
ardalan mansouri (2 года назад)
It is actually Fok1 (or capital i as a Greek number instead of "1"). For more information regarding its discovery please go to the following link published by the first researcher discovered it: http://www.ncbi.nlm.nih.gov/pubmed/6282705

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