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The CRISPR-Cas9 System Part 1

472 ratings | 65157 views
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering.
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Text Comments (79)
The One (9 days ago)
My ears bleed every time you say a word with a "S" in it .
Ben Giddings (1 month ago)
you the man THANKS !
doug chang (2 months ago)
Love this dude.
ShakespeareCafe (3 months ago)
Watch it at 2.0x speed and it's like intellectual music...if you can't keep up mentally challenge yourself...most videos on youtube are way too slow
Shikha Dubey (3 months ago)
Great explation sir again.
kindlygentleman (4 months ago)
Thanks so much. Amazing videos
Lucy Kelly (6 months ago)
CRISPR is the triumph of rape. No consideration is being given to consent issues. Genes make people and people have preferences. People refuse intimacy with some other people. No consideration is being given to the preferences of embodied genes. Rapist genes are assuming full control of nonrapist genes. This MUST be stopped! This will be one of the reasons for the return of Jesus. To stop the rape!
murxell (7 months ago)
Fantastic explanation, thank you!
Denis Santerre (7 months ago)
I watched many videos about CRISPR before viewing this series and kept kept saying to myself "whaaaat???" Yes the disfluencies can be bothersome but if you really want to understand CRISPR you will only need to watch the series once. It is very detailed but very simple to understand. Perhaps that's why instead of saying to myself "whaaat??" I was saying "oKAAAY."
דני רזניצקי (10 months ago)
very helpful thanks a million!
דני רזניצקי (10 months ago)
thank you !
sabaafzal11 (11 months ago)
BRITISH ARE inherently sick and schizophrenic. 😯
mariya aziz (1 year ago)
informative
Ferroxa (1 year ago)
Great job explaining CRISPR/Cas9 with drawings. I can wait to start applying it to my model system!
nils leibrock (1 year ago)
sorry i really need to know something about it and the content and way of your presentation gets me but duuuuuude it is way better with subtitles no voice and spotify playing in the background.
caroline mangare (1 year ago)
Excellent explanation
Miriam Sadiq (1 year ago)
so helpful for my genetics exam thank you! :)
Mohammed Hajjaj (1 year ago)
ربنا يباركلك يا بن يا ابن جرسايد
LIM (1 year ago)
thanks so much! The vidoes were so helpful
Jessica Tong (1 year ago)
Is there a maximum number of viral genome fragments which can fit into the CRISPR locus? is it known how many viruses (or other pathogens) that the bacterium can be resistant to through this method of DNA insertion?
jpsousa4 (1 year ago)
The best part about your OK's is that they're inserted in your speech at regular intervals. This video could've been so very meta had you said "OK - KO" instead.
Monmize (1 year ago)
OKAY OKAY OKAY
Sam Dietterich (1 year ago)
Great video. Very helpful.
Sunny Summer (1 year ago)
This is great! Especially the colors makes it easy to follow.
kipling1957 (1 year ago)
Presumably this same bacterial defence system works against RNA viruses, their RNA having been reverse transcribed into DNA?
Thunder Flash (2 years ago)
CRISPR = Clustered Regularly Interspaced Short Palindromic Repeat
Jim Keller (2 years ago)
Funny how many posting below are put-off by his use of the word "OK" repeatedly. Meanwhile the very same individuals will say the word "like" 18 times in the next two minutes.
Evi1M4chine (1 year ago)
No they don’t. I don’t even speak the fucking language natively. Nor do I use Facebook. YOU do though, I bet.
Jim Keller (1 year ago)
+LouciousDark It;s my job.
LouciousDark (1 year ago)
Jim Keller For fucks sake stop being so legit!
Lucy Kelly (2 years ago)
Re 'Crisper', noone shd be allowed to design a human. That would be rape! Its too intimate. Its too controlling. Nature throws up random creations, making each unique and accidental, not anyone else's affair or right! We shd keep it this way, not allow anyone to claim the nonconsensual intimacy of having been the originator of another. With regard to other lifeforms, nothing not naturally occurring in any ecosystem should be allowed to be released into it, as it would disrupt it or even destroy it. With regard to livestock or pets, nothing shd be created that is not directly related to Earth's ecosystems, as organisms already in existence are the only ones which have a right to this world, and anything created must be healthy, happy, mobile, within the boundaries of nature, and well cared for.
1234 (1 year ago)
Lucy Kelly If you don't like it try and stop me :)
Jim Keller (2 years ago)
I disagree. One of "nature's" evolutes is the human brain. When we became powerful enough to invent medicines and technologies that altered the course of this planet, we became responsible for our evolution. We are no longer in a position to evolve according to environmental dictates. We have been gradually attaining control over our bodies and the Earth (for better or worse) for decades. A century ago the average human lifespan was 40. There is no reason I can see for not CONTINUING to attain the tools to ameliorate disease. Because we have such power,, it does not mean we should run from it. Instead, what we should ask is "how can we use this technology responsibly without doing more harm than good." It is an awesome responsibility, but I believe inevitable. Our intellectual; evolution has brought it into the light. We cannot simply say "ok let's stop our research here." It has never worked that way for humans in any realm of science....not that I can see.
Thomas Fester (2 years ago)
In the case of plant genetic engineering the GVO-status of Crispr/Cas- knockout-plants is contentious. I have produced a video on this topic (Crisp/Cas and the new borderline cases of plant genetic engineering ; https://www.youtube.com/watch?v=QLpA_fYXYjc).
Mr. Bond Ng (2 years ago)
Thank you for putting in so much time and effort to make things clear. I love the use of color in explanation. It helps visual learner to see things better! Thanks!
Marcos Vieira (2 years ago)
Nice videos. Thanks!
maddy (2 years ago)
THANKS SO SO SO MUCH! YOU ARE AWESOME! =)
Greg Garnner (2 years ago)
Thanks for the video, and everythings are OKAY ;)
Ranish Shrestha (2 years ago)
Thank you very much for your videos...I've learnt quite a lot from them... I appreciate how detailed your explanation is... :)
red watch (2 years ago)
Great job. Thanks.
verwulf3 (2 years ago)
Fnx!!!!!!!!!! very good video!
YouTubist666 (2 years ago)
This is an excellently presented explanation. Very well articulated. Clear. Good for me, l am pretty much a layman. Great job.
hkgs_knight 42 (2 years ago)
Why us everyone being so rude about his voice lol? I mean he spent time creating a video to explain CRIPSR to YOU and you complain about his voice! How ungrateful smh
Assalla Mazen (9 months ago)
Simply, because he is successful while they are assholes >> Thanks for the video .. it is really helpful!
John Stoll (1 year ago)
I don't care what he sounds like as long as I learn more about CRISPR.
Evi1M4chine (1 year ago)
Because on the Internet, you don’t have to look people in the eyes, nor fear the reaction. Just like overpopulation. And worse: You can’t take away anonymity either, at these population sizes, as, purely statistically, there’s at least 5000 people, who can find a, to them, valid reason to kill you, and have both the will and the means to do so. If you want to do something against that, and also fix animal suffering, you can promote contraception.
Jim Keller (2 years ago)
Yeah, most are ungrateful. Generally those who contribute nothing.
cafedeorigin (2 years ago)
ok, right
Sean Tomlinson (2 years ago)
Clustered regularly-interspaced short palindromic repeats
Charlie d'Estries (2 years ago)
Thanks for the great explanation... a few questions... where does the CRISPR repeats come from? Are the repeats from the initial virus and the viral sequences between the repeats collected from different viruses as the cell is infected over time? Does this protect the cell from all viruses, or just those viruses that have already visited?
Matt Incha (2 years ago)
The repeats are foreign DNA that was processed and integrated into the genome through a different Cas system. Its an acquired bacterial immune system so the cell has many many different repeat loci which it or its ancestors accumulated from viral infections. Each locus only protects the cell from viruses with the same 20 bp DNA sequence that it contains; whether a single locus can protect the cell from an infection from a virus that wasn't its initial source is dependent on the specificity of the locus sequence
Andrew Palfreyman (2 years ago)
Great content, impossibly faggy voice OK?
Jiaying Zheng (2 years ago)
CRISPRs stands for clustered regularly interspaced short palindromic repeats
Ape MMAN (2 years ago)
Nice job man!
Johnthadiabetic (2 years ago)
the S of CRISPR stands for spaced, as in '...inter-spaced palindromic repeats' not interspersed.
Theo Valentino (2 years ago)
+DiabeticLime S is for short, but you're right about interspaced!
Massub Tehseen (2 years ago)
I love the way you say OKAAY after every few seconds :D nice videos though, very informative. thank you :)
zahnpastacremetube (2 years ago)
Great content, only one suggestion: Keep the color coding consistent it makes it more logical ;-)
Martin KM (2 years ago)
Nice, neat, and simple explanation. Thanks a lot
AlmiirA (2 years ago)
It's so adorable how often you say "okay" ☺️. Thankyou for the clear explanation! 😄
Samantha (2 years ago)
i really wanted to watch this but couldn't because of how annoying it is... OK
LouciousDark (1 year ago)
Samantha Bray Hey don't attack me for attacking them pathetic. Lmao
Samantha (1 year ago)
Hey don't get offended and try and attack me that's pathetic lol. It's not just me that found it annoying.
Sunny Summer (1 year ago)
We understand you have a listening and comprehending problem. So Bray, you can just stray off....
anca mitchell (2 years ago)
+Samantha Bray I love the voice.
Jonodude0 (2 years ago)
+Samantha Bray You are missing out
Matthew Ferguson (2 years ago)
STOP SAYING "OKAY" EVERY THREE SECONDS! Good video though
Chan Woo Kim (2 years ago)
+Matthew Ferguson i agree with you hahaha but it is a good video.
Emilia Gianova (2 years ago)
+Jessica oo Same , its nice . Its like the coma , that lacks in general speech.
Raghu Raman (2 years ago)
Helped a lot!!!!!!!!!!! Thanks :)
Lena Asai (2 years ago)
Very helpful thank you!
Farhan Chowdhury (2 years ago)
i love this guy <3
Soshee Ananda (2 years ago)
OK so
AoiHana (3 years ago)
Clear concise and explanatory! I plan to apply CRISPR in my Masters research
Neelima Vaddadi (3 years ago)
great job! That was very helpful thank you! :)
Stephen Adeniyi (3 years ago)
Okay :)
Phoebe Walker (3 years ago)
Such straight lines!
Jay Shapiro (3 years ago)
ok... :)
bambam144 (3 years ago)
well done! very good video

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