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The CRISPR-Cas9 System Part 1

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Оценок: 487 | Просмотров: 67792
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering.
Категория: Образование
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Текстовые комментарии (80)
Suha A (4 месяца назад)
The One (5 месяцев назад)
My ears bleed every time you say a word with a "S" in it .
Ben Giddings (6 месяцев назад)
you the man THANKS !
doug chang (6 месяцев назад)
Love this dude.
ShakespeareCafe (8 месяцев назад)
Watch it at 2.0x speed and it's like intellectual music...if you can't keep up mentally challenge yourself...most videos on youtube are way too slow
Shikha Dubey (8 месяцев назад)
Great explation sir again.
kindlygentleman (8 месяцев назад)
Thanks so much. Amazing videos
Lucy Kelly (11 месяцев назад)
CRISPR is the triumph of rape. No consideration is being given to consent issues. Genes make people and people have preferences. People refuse intimacy with some other people. No consideration is being given to the preferences of embodied genes. Rapist genes are assuming full control of nonrapist genes. This MUST be stopped! This will be one of the reasons for the return of Jesus. To stop the rape!
murxell (11 месяцев назад)
Fantastic explanation, thank you!
Denis Santerre (1 год назад)
I watched many videos about CRISPR before viewing this series and kept kept saying to myself "whaaaat???" Yes the disfluencies can be bothersome but if you really want to understand CRISPR you will only need to watch the series once. It is very detailed but very simple to understand. Perhaps that's why instead of saying to myself "whaaat??" I was saying "oKAAAY."
דני רזניצקי (1 год назад)
very helpful thanks a million!
דני רזניצקי (1 год назад)
thank you !
sabaafzal11 (1 год назад)
BRITISH ARE inherently sick and schizophrenic. 😯
ماریہ ملک (1 год назад)
Ferroxa (1 год назад)
Great job explaining CRISPR/Cas9 with drawings. I can wait to start applying it to my model system!
nils leibrock (1 год назад)
sorry i really need to know something about it and the content and way of your presentation gets me but duuuuuude it is way better with subtitles no voice and spotify playing in the background.
caroline mangare (1 год назад)
Excellent explanation
Miriam Sadiq (1 год назад)
so helpful for my genetics exam thank you! :)
Mohammed Hajjaj (1 год назад)
ربنا يباركلك يا بن يا ابن جرسايد
LIM (2 года назад)
thanks so much! The vidoes were so helpful
Jessica Tong (2 года назад)
Is there a maximum number of viral genome fragments which can fit into the CRISPR locus? is it known how many viruses (or other pathogens) that the bacterium can be resistant to through this method of DNA insertion?
jpsousa4 (2 года назад)
The best part about your OK's is that they're inserted in your speech at regular intervals. This video could've been so very meta had you said "OK - KO" instead.
Monmize (2 года назад)
Sam Dietterich (2 года назад)
Great video. Very helpful.
Sunny Summer (2 года назад)
This is great! Especially the colors makes it easy to follow.
kipling1957 (2 года назад)
Presumably this same bacterial defence system works against RNA viruses, their RNA having been reverse transcribed into DNA?
Thunder Flash (2 года назад)
CRISPR = Clustered Regularly Interspaced Short Palindromic Repeat
Jim Keller (2 года назад)
Funny how many posting below are put-off by his use of the word "OK" repeatedly. Meanwhile the very same individuals will say the word "like" 18 times in the next two minutes.
Evi1M4chine (1 год назад)
No they don’t. I don’t even speak the fucking language natively. Nor do I use Facebook. YOU do though, I bet.
Jim Keller (2 года назад)
+LouciousDark It;s my job.
LouciousDark (2 года назад)
Jim Keller For fucks sake stop being so legit!
Lucy Kelly (2 года назад)
Re 'Crisper', noone shd be allowed to design a human. That would be rape! Its too intimate. Its too controlling. Nature throws up random creations, making each unique and accidental, not anyone else's affair or right! We shd keep it this way, not allow anyone to claim the nonconsensual intimacy of having been the originator of another. With regard to other lifeforms, nothing not naturally occurring in any ecosystem should be allowed to be released into it, as it would disrupt it or even destroy it. With regard to livestock or pets, nothing shd be created that is not directly related to Earth's ecosystems, as organisms already in existence are the only ones which have a right to this world, and anything created must be healthy, happy, mobile, within the boundaries of nature, and well cared for.
1234 (2 года назад)
Lucy Kelly If you don't like it try and stop me :)
Jim Keller (2 года назад)
I disagree. One of "nature's" evolutes is the human brain. When we became powerful enough to invent medicines and technologies that altered the course of this planet, we became responsible for our evolution. We are no longer in a position to evolve according to environmental dictates. We have been gradually attaining control over our bodies and the Earth (for better or worse) for decades. A century ago the average human lifespan was 40. There is no reason I can see for not CONTINUING to attain the tools to ameliorate disease. Because we have such power,, it does not mean we should run from it. Instead, what we should ask is "how can we use this technology responsibly without doing more harm than good." It is an awesome responsibility, but I believe inevitable. Our intellectual; evolution has brought it into the light. We cannot simply say "ok let's stop our research here." It has never worked that way for humans in any realm of science....not that I can see.
Thomas Fester (2 года назад)
In the case of plant genetic engineering the GVO-status of Crispr/Cas- knockout-plants is contentious. I have produced a video on this topic (Crisp/Cas and the new borderline cases of plant genetic engineering ; https://www.youtube.com/watch?v=QLpA_fYXYjc).
J N (2 года назад)
Thank you for putting in so much time and effort to make things clear. I love the use of color in explanation. It helps visual learner to see things better! Thanks!
Marcos Vieira (2 года назад)
Nice videos. Thanks!
maddy (2 года назад)
Greg Garnner (2 года назад)
Thanks for the video, and everythings are OKAY ;)
Ranish Shrestha (2 года назад)
Thank you very much for your videos...I've learnt quite a lot from them... I appreciate how detailed your explanation is... :)
red watch (2 года назад)
Great job. Thanks.
verwulf3 (2 года назад)
Fnx!!!!!!!!!! very good video!
YouTubist666 (2 года назад)
This is an excellently presented explanation. Very well articulated. Clear. Good for me, l am pretty much a layman. Great job.
hkgs_knight 42 (2 года назад)
Why us everyone being so rude about his voice lol? I mean he spent time creating a video to explain CRIPSR to YOU and you complain about his voice! How ungrateful smh
Assalla Hook (1 год назад)
Simply, because he is successful while they are assholes >> Thanks for the video .. it is really helpful!
John Stoll (1 год назад)
I don't care what he sounds like as long as I learn more about CRISPR.
Evi1M4chine (1 год назад)
Because on the Internet, you don’t have to look people in the eyes, nor fear the reaction. Just like overpopulation. And worse: You can’t take away anonymity either, at these population sizes, as, purely statistically, there’s at least 5000 people, who can find a, to them, valid reason to kill you, and have both the will and the means to do so. If you want to do something against that, and also fix animal suffering, you can promote contraception.
Jim Keller (2 года назад)
Yeah, most are ungrateful. Generally those who contribute nothing.
cafedeorigin (2 года назад)
ok, right
Sean Tomlinson (2 года назад)
Clustered regularly-interspaced short palindromic repeats
Charlie d'Estries (2 года назад)
Thanks for the great explanation... a few questions... where does the CRISPR repeats come from? Are the repeats from the initial virus and the viral sequences between the repeats collected from different viruses as the cell is infected over time? Does this protect the cell from all viruses, or just those viruses that have already visited?
Matt Incha (2 года назад)
The repeats are foreign DNA that was processed and integrated into the genome through a different Cas system. Its an acquired bacterial immune system so the cell has many many different repeat loci which it or its ancestors accumulated from viral infections. Each locus only protects the cell from viruses with the same 20 bp DNA sequence that it contains; whether a single locus can protect the cell from an infection from a virus that wasn't its initial source is dependent on the specificity of the locus sequence
Andrew Palfreyman (2 года назад)
Great content, impossibly faggy voice OK?
Jiaying Zheng (2 года назад)
CRISPRs stands for clustered regularly interspaced short palindromic repeats
Ape MMAN (2 года назад)
Nice job man!
Johnthadiabetic (3 года назад)
the S of CRISPR stands for spaced, as in '...inter-spaced palindromic repeats' not interspersed.
Theo Valentino (2 года назад)
+DiabeticLime S is for short, but you're right about interspaced!
Massub Tehseen (3 года назад)
I love the way you say OKAAY after every few seconds :D nice videos though, very informative. thank you :)
zahnpastacremetube (3 года назад)
Great content, only one suggestion: Keep the color coding consistent it makes it more logical ;-)
Martin KM (3 года назад)
Nice, neat, and simple explanation. Thanks a lot
AlmiirA (3 года назад)
It's so adorable how often you say "okay" ☺️. Thankyou for the clear explanation! 😄
Samantha (3 года назад)
i really wanted to watch this but couldn't because of how annoying it is... OK
LouciousDark (2 года назад)
Samantha Bray Hey don't attack me for attacking them pathetic. Lmao
Samantha (2 года назад)
Hey don't get offended and try and attack me that's pathetic lol. It's not just me that found it annoying.
Sunny Summer (2 года назад)
We understand you have a listening and comprehending problem. So Bray, you can just stray off....
anca mitchell (2 года назад)
+Samantha Bray I love the voice.
Jonodude0 (3 года назад)
+Samantha Bray You are missing out
Matthew Ferguson (3 года назад)
Chan Woo Kim (2 года назад)
+Matthew Ferguson i agree with you hahaha but it is a good video.
Emilia Gianova (2 года назад)
+Jessica oo Same , its nice . Its like the coma , that lacks in general speech.
Raghu Raman (3 года назад)
Helped a lot!!!!!!!!!!! Thanks :)
Lena Asai (3 года назад)
Very helpful thank you!
Farhan Chowdhury (3 года назад)
i love this guy <3
Soshee Ananda (3 года назад)
OK so
AoiHana (3 года назад)
Clear concise and explanatory! I plan to apply CRISPR in my Masters research
Neelima Vaddadi (3 года назад)
great job! That was very helpful thank you! :)
Stephen Adeniyi (3 года назад)
Okay :)
Phoebe Walker (3 года назад)
Such straight lines!
Jay Shapiro (3 года назад)
ok... :)
bambam144 (3 года назад)
well done! very good video

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